If it's just a throwaway line, I think the impact of a portion of the audience not getting it is negligible. If you have to explain it, through your character's exposition, for most people to get it, I'd say it's probably a bit clunky. Unless you specifically want to convey that this character is someone geeky, who likes goofy eighties films, in which case the fact it's super-niche works in its favour.
If you used it without explaining the reference, anyone it niggles at would probably
Google it, and that doesn't reveal the source of the line, which probably speaks to how iconic / well known it is. Anyone who saw the film might remember, and everyone else probably won't, but that leaves the average reader with the nagging feeling that they're not understanding, or with the sense that your characters themselves are weird. Like, that line makes sense when you know the character is a tomato turned human... Okay, scratch that, the line *still* doesn't make sense when you know the character was a tomato, not unless you're on board with all the rest of the film, which is pretty screwball. But it might just make your readers think your character is as weird as a tomato-girl, which is probably also not what you want.